Application of a clinical assay for staging and prognosis of prostate cancer diagnosed in needle core biopsy specimens

ABSTRACT: Molecular and genomic analysis of microscopic quantities of tumor from formalin-fixed and paraffin-embedded (FFPE) biopsies has many unique challenges. Here we evaluated the feasibility of obtaining transcriptome-wide RNA expression to measure prognostic classifiers from diagnostic prostate needle core biopsies. 158 samples from diagnostic needle core biopsies (Bx) and radical prostatectomies (RP) were collected from 33 patients at three hospitals, each patient provided up to 6 tumor and benign samples. Genome-wide transcriptomic profiles were generated using Affymetrix Human Exon arrays for comparison of gene expression alterations and prognostic signatures between the Bx and RP samples. For 23 patients from UCSF and CSMC, six prostate tissue samples were obtained from each patient: tumo

ORGANISM(S): Homo sapiens

SUBMITTER: Elai Davicioni 

PROVIDER: E-GEOD-72220 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress