Endothelial NOTCH1 is suppressed by circulating lipids and antagonizes inflammation during atherosclerosis

ABSTRACT: While much progress has been made in identifying the mechanisms that trigger endothelial activation and inflammatory cell recruitment during atherosclerosis, less is known about the intrinsic pathways that counteract these events. Here we identified NOTCH1 as an antagonist of endothelial cell activation. NOTCH1 was constitutively expressed by adult arterial endothelium, but levels were significantly reduced by high fat diet. Furthermore, treatment of human aortic endothelial cells (HAEC) with inflammatory lipids (Ox-PAPC) and pro-inflammatory cytokines (TNFalpha and IL1beta) decreased Notch1 expression and signaling in vitro through a mechanism that requires STAT3 activation. Reduction of NOTCH1 in HAEC by siRNA, in the absence of inflammatory lipids or cytokines, increased inflammatory mo

ORGANISM(S): Homo sapiens

SUBMITTER: Anais Briot 

PROVIDER: E-GEOD-72633 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress