CAF-1 safeguards somatic cell identity during factor-induced reprogramming

ABSTRACT: Cellular differentiation involves profound changes in the chromatic landscape, yet the mechanisms by which somatic cell identity is subsequently maintained remain incompletely understood. To further elucidate regulatory pathways that safeguard the somatic state, we performed two comprehensive RNAi screens targeting chromatin factors during transcription factor-mediated reprogramming of mouse fibroblasts to induced pluripotent stem cells (iPSCs). Remarkably, subunits of the chromatin assembly factor-1 (CAF-1) complex emerged as the most prominent hits from both screens, followed by modulators of lysine sumoylation and heterochromatin maintenance. Suppression of CAF-1 increased reprogramming efficiency by several orders of magnitude and facilitated iPSC formation in as little as 4 days. Mec

ORGANISM(S): Mus musculus

SUBMITTER: Peter Park 

PROVIDER: E-GEOD-72741 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress