Transcriptomic analysis of Clostridium acetobutylicum biofilm cells in repeated-batch fermentation

ABSTRACT: Previously, we performed DNA array-based transcriptomic analysis of Clostridium acetobutylicum biofilm adsorbed onto fibrous matrix in batch fermentation. Here, to further shed light on the transcriptomic modulation of maturing Clostridium acetobutylicum biofilm, we performed the DNA array-based transcriptomic analysis in repeated-batch fermentation. Significant time course changes in expression levels were observed for the genes involved in amino acid metabolism, oligopeptide ABC transporter, nitrogen fixation, and various other processes. Repeated-batch fermentation was carried out in 2-L stainless steel columns packed with 40 g of cotton towel ?cut into pieces?approximately 3 cm × 5 cm) containing 1.5 L of P2 medium. Medium circulation rate was maintained at 35 mL/min via a peristaltic

ORGANISM(S): Clostridium acetobutylicum

SUBMITTER: Dong Liu 

PROVIDER: E-GEOD-72765 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress