Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expanding the CTD code: methylation of non-consensus Lysine residues marks early transcription in mammalian cells

ABSTRACT: Dynamic post-translational modification of RNA polymerase II (RNAPII) coordinates the co-transcriptional recruitment of enzymatic complexes that regulate chromatin states and co-transcriptional processing of nascent RNA. Extensive phosphorylation of serine residues occurs at the structurally-disordered C-terminal domain (CTD) of the largest RNAPII subunit, which is composed of multiple heptapeptide repeats with consensus sequence Y1-S2-P3-T4-S5-P6-S7. Serine-5 and Serine-7 phosphorylation mark transcription initiation, whereas Serine-2 phosphorylation coincides with productive elongation. In vertebrates, the CTD has eight non-canonical substitutions of Serine-7 into Lysine-7, which can be acetylated (K7ac). Here, we describe for the first time mono- and di-methylation of CTD Lysine-7 residues (K7me1 and K7me2). K7me1 and K7me2 are observed during the earliest transcription stages and precede or accompany Serine-5 and Serine-7 phosphorylation. Genome wide mapping of 2 novel RNAPII post-translational modifications (CTD-K7me1 and CTD-K7me2) in mouse ES cells.

ORGANISM(S): Mus musculus

SUBMITTER: Elena Torlai Triglia 

PROVIDER: E-GEOD-72876 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Methylation of RNA polymerase II non-consensus Lysine residues marks early transcription in mammalian cells.

Dias João D JD   Rito Tiago T   Torlai Triglia Elena E   Kukalev Alexander A   Ferrai Carmelo C   Chotalia Mita M   Brookes Emily E   Kimura Hiroshi H   Pombo Ana A  

eLife 20151219

Dynamic post-translational modification of RNA polymerase II (RNAPII) coordinates the co-transcriptional recruitment of enzymatic complexes that regulate chromatin states and processing of nascent RNA. Extensive phosphorylation of serine residues at the largest RNAPII subunit occurs at its structurally-disordered C-terminal domain (CTD), which is composed of multiple heptapeptide repeats with consensus sequence Y1-S2-P3-T4-S5-P6-S7. Serine-5 and Serine-7 phosphorylation mark transcription initia  ...[more]

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