Effects of agouti overexpression in the brain and skin transcriptome of zebrafish

ABSTRACT: Twenty transgenic fish overexpressing agouti-signaling protein (ASIP, Ceinos et al., Pigment Cell and Melanoma Research 28:196-209, 2015) and wild type (WT) zebrafish were placed in 6-liter tanks and acclimated during 30 days prior to sampling to minimize physiological differences. Animals were sacrificed and the brain+pituitary and the skin dissected under the stereo microscope, placed in trizol and stored at -80C until RNA extraction. Six biological replicates (3 females and 3 males) of each tissue (skin and brain) of each group (ASIP vs WT) were analyzed in a total of 6 slides containing 4 microarrays in the 4 × 44 k format. Global gene expression analyses were performed to search for both sex-dependent and transgene-dependent effects as well as the interaction between both factors (se

ORGANISM(S): Danio rerio

SUBMITTER: Aleksei Krasnov 

PROVIDER: E-GEOD-72908 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress