Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Urinary Extracellular Vesicles RNA Profile in Clear Cell Renal Cell Carcinoma as Biomarker

ABSTRACT: Background: Renal cell carcinoma (RCC) accounts for about 2% of all cancers. Renal biopsy is the gold standard among the diagnostic tools, but it is invasive and not suitable for all patients. Therefore, new reliable and non-invasive biomarkers for ccRCC detection are required. Secretion of extracellular vesicles (EVs), containing RNA molecules that can be transferred between cells, seems to be a general characteristic of malignant transformation. Consistently, cancer-derived EVs are enriched in the blood, urine and various malignant effusions of cancer patients. Therefore, urinary samples can be a non-invasive approach for discovering diagnostic biomarkers. Methods: We enrolled 33 clear-cell RCC (ccRCC) patients and 22 healthy subjects (HS), age and sex-matched, for urine collection and extracellular vesicles isolation by differential centrifugation. Transcriptional profiles of urinary EVs from 12 patients with ccRCC and 11 HS were generated using the Illumina HumanHT-12 v4 BeadChip oligonucleotide arrays. Microarray analysis led to the identification of RNA that were then validated using RT-qPCR. Results: We showed for the first time that urinary exosomal shuttle RNA (esRNA) was significantly different in ccRCC patients compared to HS and we identified three EVs esRNA involved in the tumor biology that are potentially suitable as non-invasive biomarkers. GSTA1, CEBPA and PCBD1 RNA levels decreased in urinary EVs of patients compared to HS. After 1 month post-operation, the levels of RNA increased to reach the normal level. Conclusions: This study suggests, for the first time, the potential use of the RNA content of urinary EVs to provide a non-invasive first step to diagnose the ccRCC. Total RNA obtained from urinary extracellular vesicles isolated from ccRCC patients and healthy subjects.

ORGANISM(S): Homo sapiens

SUBMITTER: Fabio Sallustio 

PROVIDER: E-GEOD-72922 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml

Similar Datasets

Unknown Urinary Extracellular Vesicles RNA Profile in Clear Cell Renal Cell Carcinoma as Biomarker
2016-09-01 | GSE72922 | GEO
Transcriptomics RNA-associated to Urinary Vesicles as a Suitable Non-invasive Source for Diagnostic Purposes in Bladder Cancer
2014-01-29 | E-GEOD-51843 | biostudies-arrayexpress
Unknown A Pilot Study on the Potential of RNA-Associated to Urinary Vesicles as a Suitable Non-Invasive Source for Diagnostic Purposes in Bladder Cancer
2014-01-29 | GSE51843 | GEO
Unknown Cell and microvesicle urine microRNA deep sequencing profiles from healthy individuals
2016-01-28 | GSE72183 | GEO
Proteomics Glioblastoma biomarkers in urinary extracellular vesicles reveal the potential for a ‘liquid gold’ biopsy
2024-04-18 | PXD046511 | Pride
Unknown Identifying urinary RNA from prospective urine as non-invasive biomarkers for kidney transplant patients
2019-03-16 | GSE128347 | GEO
Unknown Identifying urinary RNA from biopsy associated urine as non-invasive biomarkers for kidney transplant patients
2019-03-16 | GSE128348 | GEO
Unknown RNA profiles in serum and urine extracellular vesicles of patients with cholangiocarcinoma mimicking tumor expression: novel liquid biopsy approach
2020-05-18 | GSE144521 | GEO
Proteomics Changes in the urinary extracellular vesicle proteome are associated with nephronophthisis-related ciliopathies
2018-08-06 | PXD009784 | Pride
Proteomics A urine storage protocol that is feasible with at home self-sampling for extracellular vesicle proteomics
2022-05-04 | PXD024324 | Pride