Immediate dysfunction of vaccine-elicited CD8+ T cells primed in the absence of CD4+ T cells

ABSTRACT: CD4+ T cell help is critical for optimal CD8+ T cell expansion after priming in many experimental systems. However, a role for CD4+ T cells in regulating the initial steps of CD8+ T cell effector differentiation is not well established. Here we demonstrate that absence of CD4+ T cells at the time of replication-incompetent adenovirus vector immunization of C57BL/6 mice led to immediate CD8+ T cell dysfunction characteristic of exhaustion at the first detectable timepoints as well as impaired expansion of antigen-specific CD8+ T cells. The absence of CD4+ T cell help resulted in antigen-specific CD8+ T cells that had reduced ex vivo cytotoxicity and decreased capacity to produce IFN-γ and TNF-α. CD8+ T cells primed in the absence of CD4+ T cells expressed elevated levels of the inhibitory

ORGANISM(S): Mus musculus

SUBMITTER: Nicholas Provine 

PROVIDER: E-GEOD-73001 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress