RNA-sequencing based analysis of Listeria monocytogenes 10403S::ΔBCHL Prha-sigH and ΔBCHL Prha

ABSTRACT: In this study, RNA-seq was used to compare the transcriptomes of Listeria monocytogenes 10403S::ΔBCHL Prha-sigH and ΔBCHL Prha. RNA-seq was performed on ΔBCHL Prha-sigH and ΔBCHL Prha RNA samples representing three independent biological replicates at log phase in Brain Heart Infusion (BHI) broth under rhamnose induction. Indexed and purified cDNA libraries (6 libraries including 3 replicates for 2 strains) were loaded together onto an independent flow cell without any other samples; sequencing was carried out by running Hiseq 2500 (single-end, 150-bp per read). Reads alignment was carried out using the Burrows-Wheeler Aligner (BWA). Differential expression of genes in different strains was statistically assessed using the BaySeq method. To identify sigH-dependent promoters, a new m

ORGANISM(S): Listeria monocytogenes 10403S

SUBMITTER: Martin Wiedmann 

PROVIDER: E-GEOD-73008 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress