Transcription of mammalian cis-regulatory elements is restrained by actively enforced early termination [additional RNA]

ABSTRACT: Upon recruitment to active enhancers and promoters, RNA polymerase II (Pol_II) generates short non-coding transcripts of unclear function. The mechanisms that control the length and the amount of ncRNAs generated by cis-regulatory elements are largely unknown. Here, we show that the adapter protein WDR82 and its associated complexes actively limit such non-coding transcription. WDR82 targets the SET1/COMPASS H3K4 methyltransferase and the nuclear Protein Phosphatase 1 (PP1) complexes to the initiating Pol_II. WDR82 and PP1 also interact with components of the transcriptional termination and RNA processing machineries. Depletion of WDR82, SET1 or the PP1 subunit required for its nuclear import caused distinct but overlapping transcription termination defects at highly expressed genes, activ

ORGANISM(S): Mus musculus

SUBMITTER: Iros Barozzi 

PROVIDER: E-GEOD-73021 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress