RNAseq analysis of impact of PNN on gene expression and alternative splicing in corneal epithelial cells

ABSTRACT: The specialized corneal epithelium requires differentiated properties, specific for its role at the anterior surface of the eye, thus tight maintenance of the differentiated qualities of the corneal epithelial is essential. Our studies have focused on pinin (PNN), an exon junction component (EJC) that has dramatic implications on corneal epithelial cell differentiation and may act as a stabilizer of the corneal epithelial cell phenotype. Our studies revealed that PNN is involved in both transcriptional repression complexes and the spliceosomal complexes, placing PNN at the fulcrum between chromatin and mRNA splicing. Transcriptome analysis of PNN-knockdown cells revealed clear and reproducible alterations in transcript profiles and splicing patterns of a subset of genes that would signif

ORGANISM(S): Homo sapiens

SUBMITTER: Stephen Sugrue 

PROVIDER: E-GEOD-73060 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress