Constitutive stringent response restores viability of Bacillus subtilis lacking Structural Maintenance of Chromosome protein

ABSTRACT: Bacillus subtilis mutants lacking the SMC-ScpAB complex are severely impaired for chromosome condensation and partitioning, DNA repair, and cells are not viable under standard laboratory conditions. We isolated suppressor mutations that restored the capacity of a smc deletion mutant (Δsmc) to grow under standard conditions. These suppressor mutations reduced chromosome segregation defects and abrogated hypersensitivity to gyrase inhibitors of Δsmc. Three suppressor mutations were mapped in genes involved in tRNA aminoacylation and maturation pathways. A transcriptomic survey of isolated suppressor mutations pointed to a potential link between suppression of Δsmc and induction of the stringent response. This link was confirmed by (p)ppGpp quantification which indicated a constitutive induct

ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168

SUBMITTER: Etienne Dervyn 

PROVIDER: E-GEOD-73315 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress