Transcription profiling of Arabidopsis response to aluminum stress

ABSTRACT: To better understand the mechanisms involved in aluminum (Al) toxicity and tolerance in plants, microarray technology was used to evaluate changes in gene expression in Arabidopsis thaliana under Al stress. With the use of Affymetrix Arabidopsis ATH1 Genechip, a comparison of RNA expression profiles was made between control and Al-treated Arabidopsis seedlings. A total of 256 genes were identified as Al-responsive. Ninety-four genes were shown to be up-regulated and 162 were down-regulated; comprising 1.1% of the 24,000 Arabidopsis genes. Real-time RT-PCR was used to confirm the microarray data. RNAs associated with known protein functions were classified into categories. The analysis showed that a large number of transcription factors and several putative signaling components were up-regu

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Shirlean Goodwin 

PROVIDER: E-GEOD-7334 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress