Single cell RNA-Seq of Hira-KO and control MII oocytes [Zp3-Cre]

ABSTRACT: The integrity of chromatin, which provides a dynamic template for all DNA-related processes in eukaryotes, is maintained through replication-dependent and -independent assembly pathways. To address the role of replication-independent histone deposition, we deleted the histone H3.3 chaperone Hira in developing mouse oocytes. We show that chromatin of non-replicative developing oocytes is highly dynamic, and that lack of continuous H3.3/H4 deposition alters chromatin structure, resulting in increased DNase I sensitivity, the accumulation of DNA damage, and, ultimately, a severe fertility phenotype. On the molecular level, abnormal chromatin structure leads to a dramatic decrease in the dynamic range of gene expression, the appearance of spurious transcripts, and inefficient de novo DNA methy

ORGANISM(S): Mus musculus

SUBMITTER: Gopuraja Dharmalingam 

PROVIDER: E-GEOD-73381 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress