Global Transcriptional profiling of bone marrow derived hepatocytes
Ontology highlight
ABSTRACT: Whole genome expression of bone marrow deived hepatocytes after 1 and 5 months of transplantation are compared with that of primary hepatocytes and Lin- BM cells Agilent one-color experiment,Organism:Mouse, Agilent Whole Genome Mouse 8x60k (AMADID: 028005 & 026986) , Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)
Project description:Transcription profiling of E. coli MG1655, representing the effect of G181D/R258C NusA in comparison to WT NusA Agilent one-color experiment,Organism: Escherichia coli ,Agilent Custom Escherichia coli Gene Expression Microarray 8x60K (AMADID: 48792) designed by Genotypic Technology Private Limited. , Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)
Project description:The bTB research programme is centred on identifying the genetic basis for resistance against bovine tuberculosis and towards diagnostics Agilent Whole Genome Bovine 8x60k Gene Expression (AMADID: 29411) , Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)
Project description:Microarray analysis of the gill tissues of WSSV infected shrimp (P. monodon) at different time intervals 6 hrs, 24 hrs, 48 hrs and moribund stage of post WSSV infection was carried out to identify differentially expressed genes in response to WSSV infection. The shrimps in WSSV challenege experiment were challenged through intra muscular route with known concentration of virus. The important immune genes identified would be further characterized by sequence analysis and gene expression profile would be validated by real time PCR One-color experiment,Organism: Penaeus monodon, Custom Penaeus monodon (Black Tiger Shrimp) 8x60k designed by Genotypic Technology Private Limited (AMADID: 041733), Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)
Project description:To further identify gene expression signatures in the niche cells (CD45 negative) during proliferation of Hematopoietic stem cells (LSK), we employed mice whole genome (60K) microarray expression profiling as a discovery platform to identify the up-regulated and down-regulated genes of the niche. [Samples A-D] In this experiment a physiological stress model was created where the recipient mice were subjected to sub lethal radiation (700 CGy) following a transplantation of 30,000 LSK cells (HSCs). Bone marrow cells were isolated on day 0 (before transplantation) and day 10 (post transplantation of 30,000 LSK cells when maximum proliferation of HSCs was observed). Donor HSCs was sorted by FACS following RNA isolation and cDNA synthesis followed by single color global gene expression analysis. Agilent one-color experiment,Organism:Mouse, Agilent Whole Genome Mouse 8x60k (AMADID: 26986) , Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)
Project description:Sprague Dawley rats were exposed to Hypobaric Hypoxia for 1, 3 and 7 days followed isolation of Hippocampus. Microarray was performed utilizing RNA isolated from these samples. Each group included 5 animals. Agilent Rat Gene Expression 8X60K (AMADID: 028279) , Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)
Project description:Wide inter-individual variation in terms of outcome and toxic side effects of treatment exist among patients with AML receiving chemotherapy with cytarabine (Ara-C) and daunorubicin (Dnr). Drug resistance and relapse are considered major causes of treatment failure. Gene expression profiling was undertaken to address possible mechanisms of Ara-C/Dnr resistance. Based on ex vivo Ara-C cytotoxicity at diagnosis, Ara-C sensitive (IC50 <3uM AraC) and Dnr sensitive samples (IC50 < 0.5 uM) (5 samples each) were included for microarray analysis. These were compared with the samples which were drug resistant ex vivo at diagnosis. Our microarray experiment resulted in indentifying differentially expressed genes under ex vivo Ara-C sensitive as well as Dnr sensitive samples compared to ex vivo Drug resistant samples. One-color experiment,Organism: Homo sapiens, Custom Human Whole Genome 8x60k Array designed by Genotypic Technology Private Limited (AMADID: 27114), Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)
Project description:Although a lot is known about polyamines (PAs) and their functions, scientists have not yet been able to completely understand and elucidate their mode of action and interactive molecular partners. An attempt has been made in this direction by RNAi-mediated down-regulation of a key biosynthetic gene in PA metabolic pathway, ornithine decarboxylase (ODC), under a constitutive promoter and to correlate various morphological and physiological abnormalities observed in response to reduced PA titres with a genome wide transcriptome analysis of ODC-knockdown tobacco RNAi line. The analysis has not only thrown up a number of candidate genes whose expression could be directly or indirectly affected by cellular PA levels but has also been very informative with regards to the regulation of PA pathway. Tobacco wild-type and transgenic lines were grown under the culture room conditions of 16 h light at 25°C Agilent Tobacco Gene Expression 4x44k K (AMADID: 021113) , Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)
Project description:The Arabidopsis seeds of Col-0 and pad2.1 were grown for three weeks and were further placed in filter paper for 5 min at 4°C and then transferred to 1/2MS media with 30% PEG at 4°C for an additional 6 hours; the leaves were collected for RNA isolation. Trancriptomic profiling of the combined stress treated Col-0 and pad2.1 indicated downregulation of various abiotic and oxidative stress responsive genes in pad2.1 in response to combined stress treatment. Agilent one-color experiment,Organism: Arabidopsis thaliana ,Agilent Custom Arabidopsis 8x60k Microarray designed by Genotypic Technology Private Limited (AMADID: 48015)
Project description:mRNA Transcripts expression profile of Plasmodium berghei infected host HepG2 cells with and without SUMO1 over expression. To check the changes in the transcript profiles upon SUMO1 over expression which is responsible for Plasmodium berghei parasite's growth arrest in host HepG2 cells. Agilent one-color experiment, Organism: Homo sapiens, Custom Agilent 8x60k Human Whole Genome Microarray Gene expression (AMADID: 039494), Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442).
Project description:The current study aims to study change in gene expression profile of miltefosine resistance L. donovani with respect to miltefosine responsive L. donovani. Agilent one-color experiment,Organism: Leishmania donovani, Microarray GeneExpression 8x15k designed by Genotypic Technology Private Limited. (AMADID: 035638)