Transcription profiling of human neuroblastoma cell lines using a dene-resolution analysis method of DNA copy number variation using oligonucleotide expression microarrays

ABSTRACT: We present a novel method of using commercial oligonucleotide expression microarrays for aCGH, enabling DNA copy number measurements and expression profiles to be combined using the same platform. This method yields aCGH data from genomic DNA without complexity reduction at a median resolution of approximately 17,500 base pairs. Due to the well-defined nature of oligonucleotide probes, DNA amplification and deletion can be defined at the level of individual genes and can easily be combined with gene expression data. Experiment Overall Design: Genomic DNA isolated from peripheral blood samples and from Neuroblastoma cell lines was fragmented by DNAseI and biotin labeled by terminal transferase. Labeled DNAs were hybridized to U133plus2.0 GeneChips (Affymetrix). Hybridization and other condi

ORGANISM(S): Homo sapiens

SUBMITTER: David Lawrence Newsom 

PROVIDER: E-GEOD-7364 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress