Transcription factor trapping by RNA in gene regulatory elements (RNA-Seq)

ABSTRACT: Transcription factors (TFs) bind specific sequences in promoter-proximal and distal DNA elements in order to regulate gene transcription. RNA is transcribed from both promoter-proximal and distal DNA elements, and some DNA-binding TFs have also been shown to bind RNA. These obsevations led us to postulate that RNA transcribed from regulatory elements contributes to stable TF occupancy at these regulatory elements. We show here that the ubiquitously expressed TF YY1 binds to both proximal and distal regulatory elements and to the RNA species associated with these elements near active genes in embryonic stem cells. Inhibition of transcription from these elements reduces YY1 occupancy. In contrast, tethering of RNA species near YY1 DNA binding sites enhances YY1 occupancy. We propose th

ORGANISM(S): Mus musculus

SUBMITTER: Richard Young 

PROVIDER: E-GEOD-73859 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress