Alternative cleavage and polyadenylation in spermatogenesis coordinates chromatin regulation and post-transcriptional control

ABSTRACT: Most mammalian genes display alternative cleavage and polyadenylation (APA). Previous studies have indicated preferential expression of APA isoforms with short 3’UTRs in testes. Here we show widespread shortening of 3’UTR by APA during the first wave of spermatogenesis in mouse, with 3’UTRs being the shortest in spermatids. Shortening of 3’UTR eliminates destabilizing elements, such as U-rich elements and transposable elements, which appear to be highly potent for transcript elimination during spermatogenesis. We additionally found widespread regulation of APA in introns and global activation of upstream antisense transcripts during spermatogenesis. Interestingly, genes that display 3’UTR shortening tend to have higher levels of H3K4me3, consistent with the open chromatin feature

ORGANISM(S): Mus musculus

SUBMITTER: Wencheng Li 

PROVIDER: E-GEOD-73973 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress