PACAP dependency of genes regulated in adrenal gland

ABSTRACT: We investigated the effects of PACAP knock out on the transcriptosome profile of adrenal gland. A 35,546-clone mouse cDNA microarray was used to investigate transcriptional changes Keywords: transcript identification design In the hybridization method employed, equal amounts (2-5 ug/15.5 ul) of total RNA obtained from adrenal gland of wild type and PACAP KO mice groups were separately labeled with two different fluorescent dyes (Cy3 or Cy5) and applied on the same NIMH Mouse 36K cDNA microarray chip containing 13217 distinct Entrez gene IDs (formerly known as LocusLink) and 15888 distinct unigene IDs, the remainders are incompletely annotated IMAGE clones. Probe preparation and hybridization were performed as follows. The total RNA (15-50 ug in 15.5 ul) was incubated with amine-modified ra

ORGANISM(S): Mus musculus

SUBMITTER: Babru Samal 

PROVIDER: E-GEOD-7405 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress