Dynamics of chromatin maturation after genome replication

ABSTRACT: Replication of the eukaryotic genome occurs in the context of chromatin, a nucleoprotein packaging state consisting of repeating nucleosomes. Chromatin is commonly thought to carry epigenetic information from one generation to the next, although it is unclear how such information survives the disruptions of nucleosomal architecture that occur during genomic replication. Here, we sought to directly measure a key aspect of chromatin structure dynamics during replication – how rapidly nucleosome positions are established on the newly-replicated daughter genomes. By isolating newly-synthesized DNA marked with the nucleotide analogue EdU, we characterize nucleosome positions on both daughter genomes of budding yeast during a time course of chromatin maturation. We find that nucleosomes rapidl

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Marta Radman-Livaja 

PROVIDER: E-GEOD-74090 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress