Systematic discovery of cap-independent translation sequences in human and viral genomesÂ

ABSTRACT: To investigate gene specificity at the level of translation in both the human genome and viruses we devised a high-throughput bicistronic assay to quantify cap-independent translation. We uncover thousands of novel cap-independent translation sequences and provide insights on the landscape of translational regulation in both human and viruses. We find extensive translational elements in the 3’ untranslated region (3’UTR) of human transcripts and the polyprotein region of un-capped RNA viruses. Through the characterization of regulatory elements underlying cap-independent translation activity we identify potential mechanisms of secondary structure, short sequence motif and base-pairing with the 18S rRNA. Furthermore, we systematically map the 18S rRNA regions for which reverse complemen

ORGANISM(S): synthetic construct

SUBMITTER: Shira Weingarten-Gabbay 

PROVIDER: E-GEOD-74277 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress