Boolean modeling reveals the necessity of transcriptional regulation for bistablity in PC12 Cell Differentiation

ABSTRACT: The nerve growth factor NGF has been shown to cause cell fate decisions towards either differentiation or proliferation depending on the relative activity of downstream pERK, pAKT or pJNK signaling. However, how these protein signals are translated into and fed back from transcriptional activity to complete cellular differentiation over a time span of hours to days is still an open question. Using dynamic proteome and transcriptome data from NGF-stimulated PC12 cells over a time span of 24 hours we inferred a dynamic Boolean model capturing the temporal sequence of protein signaling, transcriptional response and subsequent autocrine feedback. Optimal model topology was achieved by introducing multiple time scales for fast cellular signaling and slower gene response and subsequent fitting

ORGANISM(S): Rattus norvegicus

SUBMITTER: Hauke Busch 

PROVIDER: E-GEOD-74327 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress