Trans-effects of sub-chromosomal duplications on DNA methylation patterns in mouse models of Down syndrome: whole genome bisulfite sequencing of cerebral samples from Dp(10)1Yey and Dp(16)1Yey mouse models.

ABSTRACT: Background: Trisomy 21 causes Down syndrome (DS), but the mechanisms by which the extra chromosome leads to deficient intellectual and immune function are not well understood. Results: Here, we profile CpG methylation in DS and control cerebral and cerebellar cortex of adults and cerebrum of fetuses. We purify neuronal and non-neuronal nuclei and T-lymphocytes and find biologically relevant genes with DS-specific methylation (DS-DM) in brain cells. Some genes show brain-specific DS-DM, while others show stronger DS-DM in T cells. Both 5-methyl-cytosine and 5-hydroxy-methyl-cytosine contribute to the DS-DM. Thirty percent of genes with DS-DM in adult brain cells also show DS-DM in fetal brains, indicating early onset of these epigenetic changes, and we find early maturation of methylation

ORGANISM(S): Mus musculus

SUBMITTER: Benjamin Tycko 

PROVIDER: E-GEOD-74505 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress