Effects of overexpression of hsa-miR-140-3p and its 5'isomiR in MCF10A and MDA-MB-231 breast cell lines

ABSTRACT: miRNAs are small noncoding RNA molecules that play an important role in post-transcriptional regulation of gene expression. Length and/or sequence variants of the same miRNA are termed isomiRs. While most isomiRs are functionally redundant compared to their canonical counterparts, so-called 5’isomiRs exhibit a shifted 5’ end and therefore a shifted seed sequence resulting in a different target spectrum. However, not much is known about the functional relevance of these isoforms. Analysis of miRNA-seq data from breast cancer cell lines identified six pairs of highly expressed miRNAs and associated 5’isomiRs. Among them, hsa-miR-140-3p was of particular interest because its 5’isomiR showed higher expression compared to the canonical miRNA annotated in miRbase. This miRNA has previou

ORGANISM(S): Homo sapiens

SUBMITTER: Cindy Körner 

PROVIDER: E-GEOD-74539 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress