Heterogeneity in early lymphoid compartments [qPCR]

ABSTRACT: In order to understand the developmental trajectories of early lymphocyte development it is crucial to prospectively isolate stage and lineage specific cells. It has become clear that early lymphoid progenitor compartments in the bone marrow are molecularly and functionally heterogeneous which warrants further investigation to refine the marker combinations used to isolate these progenitors. An initial antibody screen revealed extensive surface marker heterogeneity amongst early lymphoid progenitors. This heterogeneity was resolved using single cell gene expression assays and single cell in vitro differentiation assays, identifying marker combinations that isolate functionally distinct populations. In addition, using reporter transgenic mice we were able to identify a set of surface marker

ORGANISM(S): Mus musculus

SUBMITTER: Shamit Soneji 

PROVIDER: E-GEOD-74606 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress