Acutely isolated murine cortical astrocytes and microglia: Alzheimer's disease vs wildtype- effect of gfap-/- and vim-/-

ABSTRACT: GFAP and vimentin deficiency alters gene expression in astrocytes and microglia in wild-type mice and changes the transcriptional response of reactive glia in mouse model for Alzheimer's disease. Reactive astrocytes with an increased expression of intermediate filament (IF) proteins Glial Fibrillary Acidic Protein (GFAP) and Vimentin (VIM) surround amyloid plaques in Alzheimer's disease (AD). The functional consequences of this upregulation are unclear. To identify molecular pathways coupled to IF regulation in reactive astrocytes, and to study the interaction with microglia, we examined WT and APPswe/PS1dE9 (AD) mice lacking either GFAP, or both VIM and GFAP, and determined the transcriptome of cortical astrocytes and microglia from 15- to 18-month-old mice. Genes involved in lysosomal de

ORGANISM(S): Mus musculus

SUBMITTER: Elly Hol 

PROVIDER: E-GEOD-74614 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress