SMARCA4 regulates gene expression and higher-order chromatin structure in proliferating mammary epithelial cells

ABSTRACT: The packaging of DNA into chromatin plays an important role in transcriptional regulation and nuclear processes. Brahma related gene-1 SMARCA4 (also known as BRG1), the essential ATPase subunit of the mammalian SWI/SNF chromatin remodeling complex, uses the energy from ATP hydrolysis to disrupt nucleosomes at target regions. Although the transcriptional role of SMARCA4 at gene promoters is well-studied, less is known about its role in higher-order genome organization. SMARCA4 knockdown in human mammary epithelial MCF-10A cells resulted in 176 up-regulated genes, including many related to lipid and calcium metabolism, and 1292 down-regulated genes, some of which encode extracellular matrix (ECM) components that can exert mechanical forces and affect nuclear structure. ChIP-seq analysis of S

ORGANISM(S): Homo sapiens

SUBMITTER: Tony Imbalzano 

PROVIDER: E-GEOD-74716 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress