Derivation and differentiation of haploid human embryonic stem cells [expression array]

ABSTRACT: Diploidy is a fundamental genetic feature in mammals, in which haploid cells normally arise only as post-meiotic germ cells that serve to insure a diploid genome upon fertilization. Gamete manipulation has yielded haploid embryonic stem (ES) cells from several mammalian species, but as of yet not from humans. Here we analyzed a large collection of human parthenogenetic ES cell lines originating from haploid oocytes, leading to the successful isolation and maintenance of human ES cell lines with a normal haploid karyotype. Haploid human ES cells exhibited typical pluripotent stem cell characteristics such as self-renewal capacity and a pluripotency-specific molecular signature. Although haploid human ES cells resembled their diploid counterparts, they also displayed distinct properties incl

ORGANISM(S): Homo sapiens

SUBMITTER: Ido Sagi 

PROVIDER: E-GEOD-74805 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress