Hierarchy within the mammary STAT5-driven Wap super-enhancer

ABSTRACT: Super-enhancers comprise of dense transcription factor platforms highly enriched for active chromatin marks. A paucity of functional data led us to investigate their role in the mammary gland, an organ characterized by exceptional gene regulatory dynamics during pregnancy. ChIP-Seq for the master regulator STAT5, the glucocorticoid receptor, H3K27ac and MED1, identified 440 mammary-specific super-enhancers, half of which were associated with genes activated during pregnancy. We interrogated the Wap super-enhancer, generating mice carrying mutations in STAT5 binding sites within its three constituent enhancers. Individually, only the most distal site displayed significant enhancer activity. However, combinatorial mutations showed that the 1,000-fold gene induction relied on all enhancers. D

ORGANISM(S): Mus musculus

SUBMITTER: Michaela Willi 

PROVIDER: E-GEOD-74826 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress