Expression profiling of mendelian and non-mendelian F1 in gpr-1 overexpressing worms.

ABSTRACT: According to Mendel's laws, each parent makes an equal genetic contribution to an offspring in sexually reproducing organisms. The bipolar mitotic spindle controls the equal segregation of paternal and maternal chromosomes during the first cell division. By overexpression of a single protein, GPR-1, in the maternal strain we changed the structure of the mitotic spindle from bipolar to two monopolar spindles to segregate maternal and paternal chromosomes into different cell lineages, resulting in non-mendelian segregation for entire genomes. To follow maternal and paternal segregation of the chromosomes we used red and green histone markers respectively. By mating gpr-1-overexpressing hermaphrodites with wild-type males, mendelian F1 worms that express both markers simultaneously in all tis

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Judith Besseling 

PROVIDER: E-GEOD-74848 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress