Unknown,Transcriptomics,Genomics,Proteomics

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Analysis of gene expression in human Bronchial Alveolar Lavage Cell (BAL) cell pellets

ABSTRACT: Clinical subphenotypes of asthma are characterized by pathological, cellular, and molecular heterogeneity of the airway. Gene expression profiling of airway inflammatory cells in diseased patients by microarray analysis will provide insight into the molecular heterogeneity and support the development of hypotheses of pathological mechanisms. Moderate asthmatic patients and healthy controls were participants in the Study of the Mechanisms of Asthma (MAST, clinicaltrials.gov: NCT00595153). Severe asthmatic patients were participants in the BOBCAT study (as described in Jia G JACI 2012 and Arron JR ERJ 2014). RNA (Qiagen AllPrep with on-column DNase treatment) was isolated from Bronchial Alveolar Lavage cells for gene expression microarray analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Mark McCreary 

PROVIDER: E-GEOD-74986 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Inhibition of the kinase ITK in a mouse model of asthma reduces cell death and fails to inhibit the inflammatory response.

Sun Yonglian Y   Peng Ivan I   Webster Joshua D JD   Suto Eric E   Lesch Justin J   Wu Xiumin X   Senger Kate K   Francis George G   Barrett Kathy K   Collier Jenna L JL   Burch Jason D JD   Zhou Meijuan M   Chen Yuan Y   Chan Connie C   Eastham-Anderson Jeff J   Ngu Hai H   Li Olga O   Staton Tracy T   Havnar Charles C   Jaochico Allan A   Jackman Janet J   Jeet Surinder S   Riol-Blanco Lorena L   Wu Lawren C LC   Choy David F DF   Arron Joseph R JR   McKenzie Brent S BS   Ghilardi Nico N   Ismaili Moulay Hicham Alaoui MH   Pei Zhonghua Z   DeVoss Jason J   Austin Cary D CD   Lee Wyne P WP   Zarrin Ali A AA  

Science signaling 20151201 405

Interleukin-2 (IL-2)-inducible T cell kinase (ITK) mediates T cell receptor (TCR) signaling primarily to stimulate the production of cytokines, such as IL-4, IL-5, and IL-13, from T helper 2 (TH2) cells. Compared to wild-type mice, ITK knockout mice are resistant to asthma and exhibit reduced lung inflammation and decreased amounts of TH2-type cytokines in the bronchoalveolar lavage fluid. We found that a small-molecule selective inhibitor of ITK blocked TCR-mediated signaling in cultured TH2 ce  ...[more]