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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Etiology matters - Comparing Genomic DNA Methylation Patterns in Three Rat Models of Acquired Epilepsy (TBI – MBD-seq)

ABSTRACT: Explore DNA methylation in traumatic brain injury model of epilepsy and its relationship to gene expression. Examination of methylation changes in stimulated rats compared to sham operated animals in traumatic brain injury model of epilepsy.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Noora Puhakka

PROVIDER: E-GEOD-75119 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Etiology matters - Genomic DNA Methylation Patterns in Three Rat Models of Acquired Epilepsy.

Dębski Konrad J KJ Pitkanen Asla A Puhakka Noora N Bot Anna M AM Khurana Ishant I Harikrishnan K N KN Ziemann Mark M Kaspi Antony A El-Osta Assam A El-Osta Assam A Lukasiuk Katarzyna K Kobow Katja K

Scientific reports 20160509

This study tested the hypothesis that acquired epileptogenesis is accompanied by DNA methylation changes independent of etiology. We investigated DNA methylation and gene expression in the hippocampal CA3/dentate gyrus fields at 3 months following epileptogenic injury in three experimental models of epilepsy: focal amygdala stimulation, systemic pilocarpine injection, or lateral fluid-percussion induced traumatic brain injury (TBI) in rats. In the models studies, DNA methylation and gene express ...[more]

PMID: 27157830

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Etiology matters - Comparing Genomic DNA Methylation Patterns in Three Rat Models of Acquired Epilepsy (TBI â mRNA-seq)

Project description:Explore DNA methylation in traumatic brain injury model of epilepsy and its relationship to gene expression. Examination of expression changes in stimulated rats compared to sham operated animals in traumatic brain injury model of epilepsy.

2016-09-06 | E-GEOD-75120 | biostudies-arrayexpress

Etiology matters - Comparing Genomic DNA Methylation Patterns in Three Rat Models of Acquired Epilepsy

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Etiology matters - Comparing Genomic DNA Methylation Patterns in Three Rat Models of Acquired Epilepsy (Amygdala stimulation â MBD-seq)

Project description:Explore DNA methylation in focal amygdala stimulation model of epilepsy and its relationship to gene expression. Examination of methylation changes in stimulated rats compared to sham operated animals in focal amygdala stimulation model of epilepsy.

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Common molecular signature of two neuroprotective agents

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Project description:Transcriptome studies of brain resections from mesial temporal lobe epilepsy (mTLE) patients revealed a dysregulation of transforming growth factor (TGF)-β, interferon (IFN)-α/β and nuclear factor erythroid 2-related factor 2 (NRF2) pathways among other neuroinflammatory mechanisms. Since ubiquitin-specific proteases (USP), in particular USP15, have been shown to regulate these pathways, we hypothesized that the blockade of USP15 may provide therapeutic relief in treatment-resistant epilepsies. The intrahippocampal kainate mouse model for mTLE, an established model for pharmacoresistant epilepsy was used for validation of USP15 as a therapeutic target. Transgenic mice which constitutively lack USP15 underwent intrahippocampal kainate injections to investigate the impact of USP15 inactivation at the transcriptomic level.

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RNA-Sequencing of USP15 conditional knock-out mice injected with kainate - dataset 2

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