Principles for RNA metabolism and alternative transcription initiation within closely spaced promoters

ABSTRACT: Mammalian transcriptomes are complex and formed by extensive promoter activity. Moreover, gene promoters are largely divergent and initiate transcription of reverse-oriented promoter upstream transcripts (PROMPTs). Although PROMPTs are commonly terminated early, influenced by early polyadenylation (pA) sites, promoters often cluster so that the divergent activity of one might impact another. Here, we find that the distance between promoters strongly correlates with the expression, stability and length of their associated PROMPTs. Adjacent promoters driving divergent mRNA transcription support PROMPT formation, but due to pA site constraints, these transcripts tend to spread into the neighboring mRNA on the same strand. This mechanism to derive new alternative mRNA transcription start sites

ORGANISM(S): Homo sapiens

SUBMITTER: Aino Järvelin 

PROVIDER: E-GEOD-75183 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress