Microarray analysis profiling of the effect of cowpea phenolic extract on gene expression in bovine peripheral blood in vitro

ABSTRACT: Plant-derived secondary metabolites found in animal feed sources are beneficial for nutrition and health. Cowpea is a protein and phenolic-rich forage used as feed resource in animal system. The objective of this study was to understand the effect of cowpea secondary metabolites on gene expression in cows blood in vitro. Whole blood collected from Holstein Friesian cows (n=5) were treated with 10 ug/ml of cowpea leaf phenolic extract and untreated samples served as control. Total RNA was isolated and pooled together for microarray analysis. The Agilent one color bovine (v2) 4x44K array was used and preliminary gene expression profiles generated using Cy3 labeled cDNA from CPE-stimulated and untreated samples. Gene expression analysis revealed a total of 3170 differentially expressed genes

ORGANISM(S): Bos taurus

SUBMITTER: Mulumebet Worku 

PROVIDER: E-GEOD-75239 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress