Microsensor and transcriptome signatures of oxygen depletion in biofilms associated with chronic wounds [biofilm inoculum]

ABSTRACT: Biofilms have been implicated in delayed wound healing, although the mechanisms by which biofilms impair wound healing are poorly understood. Many species of bacteria produce exotoxins and exoenzymes that may inhibit healing. In addition, oxygen consumption by biofilms, as well as responding leukocytes, may impede wound healing. In this study, we used oxygen microsensors to measure oxygen transects through in vitro-cultured biofilms, biofilms formed in vivo within scabs from a diabetic (db/db) mouse model, and ex vivo human chronic wound specimens. The results show that oxygen levels within mouse scabs had steep gradients that reached minima ranging from 17-72 mmHg on live mice and 6.4-1.1 mmHg on euthanized mice. The oxygen gradients in the mouse scabs were similar to those observed for c

ORGANISM(S): Pseudomonas aeruginosa PAO1

SUBMITTER: Kerry Williamson 

PROVIDER: E-GEOD-75359 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress