Nuclear receptor coactivator 1 (NCOA1) is involved in the regulation of PCa cell migration via PRKD1

ABSTRACT: Due to the urgent need of new targeting strategies in PCa, AR interacting proteins should be considered. In this study we aimed to test the effect of a long-term knockdown of NCOA1, an AR coactivator, in PCa progression and metastatogenesis and whether NCOA1 could be used as a possible therapeutic target. To test the consequences of NCOA1 knockdown on proliferation, we performed by 3H thymidine incorporation assays revealing a strong reduction in castration resistant MDA PCa 2b and androgen-dependent LNCaP cells, without affecting AR negative PC3 cells. Furthermore, Boyden chamber assays revealed a strong decrease in migration and invasion upon NCOA1 knockdown. Using a cDNA microarray, we identified protein kinase D1 (PRKD1) as one prominent upregulated gene in MDA PCa 2b, which was not se

ORGANISM(S): Homo sapiens

SUBMITTER: Johannes Rainer 

PROVIDER: E-GEOD-75531 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress