Invariant TAD boundaries constrain cell type-specific looping interactions between promoters and distal elements around the CFTR locus.

ABSTRACT: Three-dimensional genome structure plays an important role in gene regulation. Globally chromosomes are organized into active and inactive compartments, while at the gene level looping interactions connect promoters to regulatory elements. Topologically Associating Domains (TADs), typically several hundred kilobases in size form an intermediate level of organization. Major questions include how TADs are formed and what their relation is with looping interactions between genes and regulatory elements. Here we performed a focused 5C analysis of a 2.8 Mb region on chromosome 7 surrounding CFTR in a panel of cell types. We find that the same TAD boundaries are present in all cell types, indicating that TADs represent a universal chromosome architecture. Further, we find that these TAD bo

ORGANISM(S): Homo sapiens

SUBMITTER: Bryan Lajoie 

PROVIDER: E-GEOD-75634 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress