Analysis of on-chip hybridization kinetics for optimization of gene expression experiments.

ABSTRACT: DNA microarray technology is a powerful tool for getting the overview of gene expression in biological samples. Although the successful application of microarray-based expression analysis was demonstrated in a number of applications, the main problem with this approach is the fact that expression levels deduced from hybridization experiments do not necessarily correlate with RNA concentrations. Moreover, oligonucleotide probes corresponding to the same gene can give different hybridization signals. Apart from cross-hybridizations and differential splicing, this could be due to secondary structures of probes or targets. In addition, for low copy genes, hybridization equilibrium may be reached after hybridization times much longer than the one commonly used (overnight, 15 hours). Thus, hybri

ORGANISM(S): Mus musculus

SUBMITTER: Luce Dauphinot 

PROVIDER: E-GEOD-7574 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress