The microRNA expression profiles changes associated with constant heat stress in porcine skeletal muscle as measured by deep sequencing

ABSTRACT: To characterize the changes of miRNAs in skeletal muscle responding to heat stress, the miRNA expression profiles of longissimus dorsi muscles of pigs raised under constant heat stress (30°C, n=8) or control temperature (22 C, n=8) for 21 days were analyzed by Illumina deep sequencing. 58 differentially expressed miRNAs were identified with 30 down-regulated and 28 up-regulated and 63 differentially expressed target genes were predicted by miRNA-mRNA joint analysis. GO and KEGG analyses showed that the genes regulated by differentially expressed miRNAs were enriched in glucose metabolism, cytoskeletal structure and function, and stress response. Real-time PCR showed that the mRNA levels of PDK4, HSP90 and DES were significantly increased whereas those of SCD and LDH-A significantly decrea

ORGANISM(S): Sus scrofa

SUBMITTER: Yue Hao 

PROVIDER: E-GEOD-75785 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress