A Temporal Switch in the Germinal Center Determines Differential Output of Memory B and Plasma Cells

ABSTRACT: There is little insight into or agreement about the signals that control differentiation of memory B cells (MBC) and long-lived plasma cells (LLPC). By performing BrdU pulse-labeling studies, we found that MBC formation preceded the formation of LLPC in an adoptive transfer immunization system, which allowed for a synchronized Ag-specific response with homogeneous Ag-receptor, yet at natural precursor frequencies. We confirmed observations in wild type (WT) mice and extended them with germinal center (GC) disruption experiments and variable region gene sequencing. We thus show that the GC response undergo a temporal switch in its output as it matures, revealing that the reaction engenders both MBC subsets with different immune effector function and, ultimately, LLPC at largely separate poi

ORGANISM(S): Mus musculus

SUBMITTER: Maria Chikina 

PROVIDER: E-GEOD-76502 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress