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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Biological characterization of virulent therapeutic bacteriophage PAK_P3 infecting Pseudomonas aeruginosa.

ABSTRACT: We have isolated and characterized several bacteriophages infecting Pseudomonas aeruginosa distantly related to Felix O1 virus and proposed they form a new subfamily named Felixounavirinae. The infectious cycle of bacteriophages belonging to this subfamily has not been studied yet in terms of gene expression. The present study reports the RNA-Seq analysis of bacteriophage PAK_P3 infecting PAK strain of P. aeruginosa. RNA profile of Host and Phage at 0min, 3.5min and 13 min after infection of Pseudomonas aeruginosa PAK strain with the Pseudomonas phage PAK P3. Three biological replicates for each time point.

ORGANISM(S): Pseudomonas aeruginosa

SUBMITTER: Monot Marc

PROVIDER: E-GEOD-76513 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Next-Generation "-omics" Approaches Reveal a Massive Alteration of Host RNA Metabolism during Bacteriophage Infection of Pseudomonas aeruginosa.

Chevallereau Anne A Blasdel Bob G BG De Smet Jeroen J Monot Marc M Zimmermann Michael M Kogadeeva Maria M Sauer Uwe U Jorth Peter P Whiteley Marvin M Debarbieux Laurent L Lavigne Rob R

PLoS genetics 20160705 7

As interest in the therapeutic and biotechnological potentials of bacteriophages has grown, so has value in understanding their basic biology. However, detailed knowledge of infection cycles has been limited to a small number of model bacteriophages, mostly infecting Escherichia coli. We present here the first analysis coupling data obtained from global next-generation approaches, RNA-Sequencing and metabolomics, to characterize interactions between the virulent bacteriophage PAK_P3 and its host ...[more]

PMID: 27380413

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