Antibody performance in ChIP-sequencing assays: From quality scores of public datasets to quantitative certification

ABSTRACT: We have established a certification system for antibodies to be used in chromatin immunoprecipitation assays coupled to massive parallel sequencing (ChIP-seq). This certification comprises a standardized ChIP procedure and the attribution of a numerical quality control indicator (QCi) to biological replicate experiments. The QCi computation is based on a universally applicable quality assessment that quantitates the global deviation of randomly sampled subsets of ChIP-seq dataset with the original genome-aligned sequence reads. Comparison with a QCi database for >28,000 ChIP-seq assays were used to attribute quality grades (ranging from ‘AAA’ to ‘DDD’) to a given dataset. In the present report we used the numerical QC system to assess the factors influencing the quality of ChIP-seq

ORGANISM(S): Homo sapiens

SUBMITTER: Marco Antonio Mendoza-Parra 

PROVIDER: E-GEOD-76618 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress