Stromal cells secrete exosomes carrying specific molecular signature involved in the support of Hematopoietic Stem and Progenitor Cells

ABSTRACT: Purpose: The study focused on the RNA content of supportive (AFT024) and non supportive (BFC012) stromal lines and their respective exosomes to analyze the molecular complexity of the Hematopoietic Stem and Progenitor Cell niche Methods: All the samples from small RNA (SR) and polyA-RNA libraries were sequenced using 1×50 bp single reads high-throughput sequencing (RNA-Seq) in single lane on Illumina HiSeq 2500. The sequence reads that passed quality filters were analyzed at the transcript isoform level with TopHat followed by Cufflinks and by Fisher's test for polyA-RNA, or with bowtie and parse tools for smallRNA (galaxy server https://lbcd41.snv.jussieu.fr/). Results: Using an optimized data analysis workflow, we mapped about 30 million sequence reads per sample to the mouse genome (b

ORGANISM(S): Mus musculus

SUBMITTER: Gregoire Stik 

PROVIDER: E-GEOD-76711 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress