Transcription profiling of mouse G9a-/- knockouts vs controls reveals G9a histone methyltransferase maintains genomic imprinting IN THE mouse placenta.

ABSTRACT: Whereas DNA methylation is essential for genomic imprinting, the importance of histone methylation in the allelic repression of imprinted genes is unclear. ‘Imprinting control regions’ (ICRs), however, are consistently marked by histone H3 K9 methylation on their DNA-methylated allele. In the placenta, the paternal silencing along the Kcnq1 domain on distal chromosome 7 also correlates with the presence of H3-K9 methylation, but imprinted repression at these genes is maintained independently of DNA methylation. To explore which histone methyltransferase (HMT) could mediate the allelic H3-K9 methylation on distal chromosome 7, and at ICRs, we generated mouse conceptuses deficient for the SET-domain protein G9a. We find that in the embryo and placenta, the differential DNA methylation at

ORGANISM(S): Mus musculus

SUBMITTER: Reiner Schulz 

PROVIDER: E-GEOD-7674 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress