Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Investigation of the molecular mechanisms underlying of herb pair Daji and Gancao with different combination designs acting on hepatocellular carcinoma ascites mice

ABSTRACT: The differentially expressed genes among the blank control, ascites mouse model, Daji (DJ)/ Gancao (GC)-synergistic treatment and DJ/GC-antagonistic treatment groups were identified to investigate the molecular mechanisms underlying the herb pair DJ and GC acting on ascites mice. In the study, 50 male Kunming mice were randomly divided into 5 groups, namely, normal control group, model group, Daji/Gancao-synergy group, Daji/Gancao-antagonism group, Daji group. Normal control group and model group were intragastrically administrated with normal saline, while other 3 groups were intragastrically administrated with corresponding doses.

ORGANISM(S): Mus musculus

SUBMITTER: Yanqiong Zhang

PROVIDER: E-GEOD-76818 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Chinese medicine is a complex system guided by traditional Chinese medicine (TCM) theories, which has proven to be especially effective in treating chronic and complex diseases. However, the underlying modes of action (MOA) are not always systematically investigated. Herein, a systematic study was designed to elucidate the multi-compound, multi-target and multi-pathway MOA of a Chinese medicine ,QSYQ, on myocardial infarction. Male Sprague Dawley rat model of myocardial infarction were administered QSYQ intragastrically for 7 days while the control group was not treated. The differentially expressed genes (DEGs) were identified from myocardial infarction rat model treated with QSYQ, followed by constructing a cardiovascular disease (CVD)-related multilevel compound-target-pathway network connecting main compounds to those DEGs supported by literature evidences and the pathways that are functionally enriched. Three conditions were compared with three replicates each: (1) sham, i.e. without left anterior descending coronary artery (LAD) ligation; (2) model, with LAD ligation; (3) QSYQ, with LAD ligation and treated with QSYQ intragastrically for 7 days, the dosage was 105.6 mg/kg once a day. Rats were sacrificed after 7 days of i.g. administration under 10% chloral hydrate anesthesia (300mg/kg). Three tissue samples on the border between infarct and non-infarct area were dissected from left ventricles of each group. The tissue samples were stored at -80M-bM-^DM-^C refrigerator. Total RNA was extracted using TRIZol Reagent (Invitrogen) and purified using RNeasy Mini kit (QIAGEN), following manufacturersM-bM-^@M-^Y protocols. RNA quality was evaluated using an Agilent 2100 Bioanalyzer and electrophoresis in 2% (w/v) agarose gels. Only RNA with RNA integrity numbers (RINs) greater than 7.0 and 28SrRNA/18S rRNA ratio greater than 0.7 was used for microarray analyses. Whole genome microarray analysis was performed using Affymetrix rat Genome 230 2.0 chips.

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Investigation of the molecular mechanisms underlying of herb pair Daji and Gancao with different combination designs acting on hepatocellular carcinoma ascites mice

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