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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-Seq data for KRAS wild-type(WT) and two mutant forms G12V and Q61H over-expressed samples with green fluorescent protein control samples using human mammary epithelial cells

ABSTRACT: The goal was to capture the transcriptional activity due to over-expression of KRAS gene. Over-expressions were validated using Western Blots. Illumina RNA-Seq technology was used to capture the downstream transcriptional activity. Reads were 101 base pairs long and single ended. An R open source package âRsubreadâ was used to align and quantify the read using UCSC hg19 annotation. The integer-based gene counts were later normalized in TPM . Profiles of gene expression were generated in cells derived from breast and used to generate a gene-expression signatures.

ORGANISM(S): Homo sapiens

SUBMITTER: Mumtahena Rahman

PROVIDER: E-GEOD-76877 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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