Single-cell RNA-seq of Ecad positive PyMT bulk tumor cells

ABSTRACT: In this study we studied the presence of tumor cells that underwent epithelial-to-mesenchymal transition within polyoma middle T antigen (PyMT) breast tumors. For this we dissociated tumors and isolated Ecad positive tumor cells by FACS sorting. We confirmed that PyMT tumors contain a small set of tumor cells that have undergone EMT in the primary tumor and that E-cadherin can be used as a marker on single cell level for mesenchymal status in this model. We isolated primary tumors from mice, dissociated the tumors and FACS-sorted for single Ecad positive tumor cells, after this we performed single cell sequencing of the cells.

ORGANISM(S): Mus musculus

SUBMITTER: Jean-Charles Boisset 

PROVIDER: E-GEOD-77107 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress