Halobacterium NRC-1 oxygen, light and sop2 perturbation

ABSTRACT: A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of sop2 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clear flask under constant standard incubator illumination, respectively. Low and high oxygen indicate shaking in a stoppered flask at 100RPM agitation or shaking in a non-stoppered flask at 220RPM, respectively. Each of the four possible pairwise environemental combinations were assayed : High-oxygen/high-light, Low-oxygen/high-light, High-oxygen/low-light and Low-oxygen/low-light. RNA extractions were performed using the Stratagene Absolutely RNA Miniprep Kit and RNA quality checked with the Ag

ORGANISM(S): Halobacterium sp. NRC-1

SUBMITTER: Min Pan 

PROVIDER: E-GEOD-7719 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress