RG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome

ABSTRACT: RNA structures are of crucial importance for biological function and gene regulation. Guanine (G)-rich sequences in RNA can assemble to form RNA G-quadruplex (rG4) structures; specific rG4s have been shown to regulate gene expression, and are associated with human diseases. However, no transcriptome-wide method has been reported to map rG4s, limiting our understanding of the rG4 structure and its relationship with function and regulation. Here we introduce a transcriptome-wide rG4 profiling method, rG4-Seq, in which the rG4-mediated reverse transcriptase stalling is read out by next-generation sequencing at nucleotide resolution. We apply this method to human RNA and report the first global map of rG4 structures for any organism. Our analysis identifies over 3,000 rG4s, and increases to ov

ORGANISM(S): Homo sapiens

SUBMITTER: Giovanni Marsico 

PROVIDER: E-GEOD-77282 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress