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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Primary human blood-derived macrophages (BDMs): control vs. infected

ABSTRACT: Transcriptional profiling of primary human blood-derived macrophages (BDMs) comparing control untreated BDMs with BDMs exposed with Streptococcus pneumoniae strain D39 (MOI 0.1 and 0.5) for 16 hours) Two-condition experiment, control BDMs vs. infected BDMs. Biological replicates: 3 control replicates, 3 infected replicates MOI 0.1, 2 infected replicates MOI 0.5).

ORGANISM(S): Homo sapiens

SUBMITTER: Jochen Wilhelm

PROVIDER: E-GEOD-77561 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MicroRNAs Constitute a Negative Feedback Loop in Streptococcus pneumoniae-Induced Macrophage Activation.

Griss Kathrin K Bertrams Wilhelm W Sittka-Stark Alexandra A Seidel Kerstin K Stielow Christina C Hippenstiel Stefan S Suttorp Norbert N Eberhardt Martin M Wilhelm Jochen J Vera Julio J Schmeck Bernd B

The Journal of infectious diseases 20160316 2

Streptococcus pneumoniae causes high mortality as a major pneumonia-inducing pathogen. In pneumonia, control of innate immunity is necessary to prevent organ damage. We assessed the role of microRNAs (miRNAs) as regulators in pneumococcal infection of human macrophages. Exposure of primary blood-derived human macrophages with pneumococci resulted in transcriptional changes in several gene clusters and a significant deregulation of 10 microRNAs. Computational network analysis retrieved miRNA-146a ...[more]

PMID: 26984146

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